SrasV1, Main, Exploration, bibRecord, 003459

Thiopurine analogues inhibit papain-like protease of severe acute respiratory syndrome coronavirus

Identifieur interne : 003459 ( Main/Exploration ); précédent : 003458; suivant : 003460

Thiopurine analogues inhibit papain-like protease of severe acute respiratory syndrome coronavirus

Auteurs : Chi-Yuan Chou [Taïwan] ; Chia-Hui Chien [Taïwan] ; Yu-San Han [Taïwan] ; Mojca Trstenjak Prebanda [Slovénie] ; Hsing-Pang Hsieh [Taïwan] ; Boris Turk [Slovénie] ; Gu-Gang Chang [Taïwan] ; XIN CHEN [Taïwan]

Source :

Biochemical pharmacology [ 0006-2952 ] ; 2008.

RBID : Pascal:08-0238691

Descripteurs français

Pascal (Inist)
- Thiopurine dérivé, Analogue, Inhibiteur, Papain, Syndrome respiratoire aigu sévère, Coronavirus, Mercaptopurine, Tioguanine, Cystéine, Anticancéreux.

English descriptors

KwdEn :
- Analog, Antineoplastic agent, Coronavirus, Cysteine, Inhibitor, Mercaptopurine, Papain, Severe acute respiratory syndrome, Thiopurine derivatives, Tioguanine.

Abstract

The papain-like protease of severe acute respiratory syndrome coronavirus (PLpro) (EC 3.4.22.46) is essential for the viral life cycle and therefore represents an important antiviral target. We have identified 6MP and 6TG as reversible and slow-binding inhibitors of SARS-CoV PLpro, which is the first report about small molecule reversible inhibitors of PLpro. The inhibition mechanism was investigated by kinetic measurements and computer docking. Both compounds are competitive, selective, and reversible inhibitors of the PLpro with K_is values ∼10 to 20 μM. A structure-function relationship study has identified the thiocarbonyl moiety of 6MP or 6TG as the active pharmacophore essential for these inhibitions, which has not been reported before. The inhibition is selective because these compounds do not exert significant inhibitory effects against other cysteine proteases, including SARS-CoV 3CLpro and several cathepsins. Thus, our results present the first potential chemical leads against SARS-CoV PLpro, which might be used as lead compounds for further optimization to enhance their potency against SARS-CoV. Both 6MP and 6TG are still used extensively in clinics, especially for children with acute lymphoblastic or myeloblastic leukemia. In light of the possible inhibition against subset of cysteine proteases, our study has emphasized the importance to study in depth these drug actions in vivo.

Affiliations:

Slovénie, Taïwan

Links toward previous steps (curation, corpus...)

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to stream PascalFrancis, to step Checkpoint: 000236
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Le document en format XML

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<front><div type="abstract" xml:lang="en">The papain-like protease of severe acute respiratory syndrome coronavirus (PLpro) (EC 3.4.22.46) is essential for the viral life cycle and therefore represents an important antiviral target. We have identified 6MP and 6TG as reversible and slow-binding inhibitors of SARS-CoV PLpro, which is the first report about small molecule reversible inhibitors of PLpro. The inhibition mechanism was investigated by kinetic measurements and computer docking. Both compounds are competitive, selective, and reversible inhibitors of the PLpro with K<sub>is</sub>
 values ∼10 to 20 μM. A structure-function relationship study has identified the thiocarbonyl moiety of 6MP or 6TG as the active pharmacophore essential for these inhibitions, which has not been reported before. The inhibition is selective because these compounds do not exert significant inhibitory effects against other cysteine proteases, including SARS-CoV 3CLpro and several cathepsins. Thus, our results present the first potential chemical leads against SARS-CoV PLpro, which might be used as lead compounds for further optimization to enhance their potency against SARS-CoV. Both 6MP and 6TG are still used extensively in clinics, especially for children with acute lymphoblastic or myeloblastic leukemia. In light of the possible inhibition against subset of cysteine proteases, our study has emphasized the importance to study in depth these drug actions in vivo.</div>
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Thiopurine analogues inhibit papain-like protease of severe acute respiratory syndrome coronavirus

Thiopurine analogues inhibit papain-like protease of severe acute respiratory syndrome coronavirus

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri